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Registros recuperados: 8
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Characterization of the hrpZ gene from Pseudomonas syringae pv. maculicolaM2 BJM
Álvarez-Mejía,César; Rodríguez-Ríos,Dalia; Hernández-Guzmán,Gustavo; López-Ramírez,Varinia; Valenzuela-Soto,Humberto; Marsch,Rodolfo.
Pseudomonas syringae pv. maculicola is a natural pathogen of members of the Brassicaceae plant family. Using a transposon-based mutagenesis strategy in Pseudomonas syringaepv. maculicola M2 (PsmM2), we conducted a genetic screen to identify mutants that were capable of growing in M9 medium supplemented with a crude extract from the leaves of Arabidopsis thaliana. A mutant containing a transposon insertion in the hrpZ gene (PsmMut8) was unable to infect adult plants from Arabidopsis thaliana or Brassica oleracea, suggesting a loss of pathogenicity. The promotorless cat reporter present in the gene trap was expressed if PsmMut8 was grown in minimal medium (M9) supplemented with the leaf extract but not if grown in normal rich medium (KB). We conducted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HrpZ; Mutant non-pathogenic; Transmid; Tn5; Phylogenetic.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300929
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Identification and phylogenetic inferences on stocks of sharks affected by the fishing industry off the Northern coast of Brazil Genet. Mol. Biol.
Rodrigues-Filho,Luis Fernando da Silva; Rocha,Tainá Carreira da; Rêgo,Péricles Sena do; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo.
The ongoing decline in abundance and diversity of shark stocks, primarily due to uncontrolled fishery exploitation, is a worldwide problem. An additional problem for the development of conservation and management programmes is the identification of species diversity within a given area, given the morphological similarities among shark species, and the typical disembarkation of processed carcasses which are almost impossible to differentiate. The main aim of the present study was to identify those shark species being exploited off northern Brazil, by using the 12S-16S molecular marker. For this, DNA sequences were obtained from 122 specimens collected on the docks and the fish market in Bragança, in the Brazilian state of Pará. We identified at least 11...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sharks; Molecular identification; Phylogenetic; MtDNA; Conservation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200031
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Molecular and morphological evidence of Alvinellidae relationships (Terebelliformia, Polychaeta, Annelida) ArchiMer
Rousset, Vincent; Rouse, Greg; Feral, Jean-pierre; Desbruyeres, Daniel; Pleijel, Fredrik.
In this study we assessed the phylogenetic relationships of the hydrothermal vent polychaete group Alvinellidae, based on parsimony analyses of combined morphological and molecular data. Morphological data were obtained from newly examined specimens and literature information of 16 terminal taxa belonging to Alvinellidae, Ampharetidae, Pectinariidae, Terebellidae, Trichobranchidae, and the outgroups Oweniidae and Sabellidae. Molecular data were based on 28S rRNA from 13 of the 16 morphological terminals (10 previously published sequences plus three new ones). The combined analysis indicated the clades ((Alvinellidae, Trichobranchidae) Pectinariidae) and (Ampharetidae, Terebellidae). Alvinellidae, Ampharetidae and Terebellidae, as currently delineated, are...
Tipo: Text Palavras-chave: Molecular; Alvinellidae; Polychaete; Phylogenetic.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-706.pdf
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Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata BABT
Esmaelizad,Majid; Niaraki,Saber Jelokhani; Fesharaki,Reza Hashemi.
The polypeptide Tams1 is an immunodominant major merozoite piroplasm surface antigen of the protozoan parasite Theileria annulata and is highly variable. In this study, the partial nucleotide (nt) sequence of the Tams1 (522 nt) gene of Iranian vaccine strain (Vaccine-ir68) recovered from an outbreak of disease in Iran was determined and compared with the corresponding sequences of eighteen previously published Tams1 genes. According to sequencing result, a novel amino acid substitution at the Tams1 region (K→Q) was found exclusively in isolate Vaccine-ir68. Genetic distance values, estimated from the sequence data, revealed striking sequence homology (approximately 99%) between Vaccine-ir68 isolate and Tunisian isolates, showing that they were same...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tams1; Theileria Annulata; Merozoite surface antigen; Phylogenetic.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600005
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Occurrence and dístríbutíon of Bemisia tabaci and Trialeurodes vaporariorum (Hemiptera: Aleyrodídae) on tomato crops in Panama Acta Agron. (Palmira)
Herrera Vásquez,José Angel; Jaén Sanjur,José Natividad; Zachrisson Salamina,Bruno Alexis; Rubio Miguélez,Luis; Barba Alvarado,Anovel Amet; Aguilera Cogley,Vidal Antonio; Valdespino,Randy Atencio; Torregrosa 0,Luis Galipienso; Mejía Franco,Luis Carlos.
Abstract The main open-field and greenhouse producer regions of tomato in Panama (Los Santos, Herrera, Veraguas and Chiriquí provinces) were surveyed to determine the occurrence and distribution of Bemisia tabaci and Trialeurodes vaporariorum during the growing seasons of 2017 and 2018. Twenty-two plots were surveyed, and DNA extracts of 112 adult whiteflies were analyzed by multiplex PCR, using a set of specific primers of B. tabaci MEAM1, B. tabaci NW, and T. vaporariorum. DNA extracts corresponding to 57 individual whiteflies (50.9 %) rendered PCR products of expected size with B. tabaci-specific primers (37 belonged to B. tabaci MEAM1 and 20 to B. tabaci NW), whereas the remaining DNA extracts corresponding to 55 individual whiteflies (49.1 %) rendered...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Altitude; PCR; Phylogenetic; Sequencing; Weather conditions; Whiteflies.
Ano: 2022 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122022000100096
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The comparative mitogenomics and phylogenetics of the two grouse-grasshoppers (Insecta, Orthoptera, Tetrigoidea) Biol. Res.
Sun,Yufang; Liu,Dianfeng; Xiao,Bo; Jiang,Guofang.
Abstract Objective This study aimed to reveal the mitochondrial genomes (mtgenomes) of Tetrix japonica and Alulatettix yunnanensis, and the phylogenetics of Orthoptera species. Methods The mtgenomes of A. yunnanensis and T. japonica were firstly sequenced and assembled through partial sequences amplification, and then the genome organization and gene arrangement were analyzed. Based on nucleotide/amino acid sequences of 13 protein-coding genes and whole mtgenomes, phylogenetic trees were established on 37 Orthoptera species and 5 outgroups, respectively. Results Except for a regulation region (A+T rich region), a total of 37 genes were found in mtgenomes of T. japonicaand A. yunnanensis, including 13 protein-coding genes, 2 ribosomal RNA genes, and...
Tipo: Journal article Palavras-chave: Mitogenome; Orthoptera Tetrigoidea; Phylogenetic.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100219
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The subjective nature of Linnaean categories and its impact in evolutionary biology and biodiversity studies Naturalis
Laurin, M..
Absolute (Linnaean) ranks are essential to rank-based nomenclature (RN), which has been used by the vast majority of systematists for the last 150 years. They are widely recognized as being subjective among taxonomists, but not necessarily in other fields. For this reason, phylogenetic nomenclature (PN) and other alternative nomenclatural systems have been developed. However, reluctance to accept alternative nomenclatural systems and continued use of higher taxa of a given Linnaean category in comparative analyses presumably reflect a lack of appreciation of the deleterious effects of the subjective nature of Linnaean categories in other biological fields, such as conservation and evolutionary biology. To make that point clearer, evolutionary models under...
Tipo: Article / Letter to the editor Palavras-chave: Biological nomenclature; Codes of nomenclature; Comparative biology; Evolution; PhyloCode; Phylogenetic; Nomenclature; 42.21.
Ano: 2010 URL: http://www.repository.naturalis.nl/record/361032
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The taxonomic status and origin of the Portuguese oyster Crassostea angulata (Lamark, 1819) ArchiMer
Batista, Frederico; Leitao, Alexandra; Huvet, Arnaud; Lapegue, Sylvie; Heurtebise, Serge; Boudry, Pierre.
The taxonomic status of the Portuguese oyster Crassostrea angulata and the Pacific oyster Crassostrea gigas has often been a matter of controversy. Based on larval shell morphology, experimental hybridisation and electrophoretic studies of enzyme polymorphism several authors have considered these two species as being synonymous. During the recent years, several genetic studies based on mitochondrial DNA and microsatellites data have provided evidences that the two taxa are genetically distinct although closely related. Furthermore, karyotype analyses have also highlighted the close genetic similarity of these taxa in comparison with other cupped oyster species. However, a comparative analysis of restriction enzymes ideograms revealed differences between...
Tipo: Text Palavras-chave: Geographical origin; Phylogenetic; Genetic; Taxonomic status; Crassostrea angulata; Oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3433.pdf
Registros recuperados: 8
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